Antigenic Cross-Reactivity between Severe Acute Respiratory Syndrome-Associated Coronavirus and Human Coronaviruses 229E and OC43.
Che, Xiao-yan 1; Qiu, Li-wen 1; Liao, Zhi-yong 1; Wang, Ya-di 1; Wen, Kun 1; Pan, Yu-xian 1; Hao, Wei 1; Mei, Ya-bo 1; Cheng, Vincent C. C. 2; Yuen, Kwok-yung 2
[Article]
Journal of Infectious Diseases.
191(12):2033-2037, June 15, 2005.
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: Cross-reactivity between antibodies to different human coronaviruses (HCoVs) has not been systematically studied. By use of Western blot analysis, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA), antigenic cross-reactivity between severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) and 2 HCoVs (229E and OC43) was demonstrated in immunized animals and human serum. In 5 of 11 and 10 of 11 patients with SARS, paired serum samples showed a >=4-fold increase in antibody titers against HCoV-229E and HCoV-OC43, respectively, by IFA. Overall, serum samples from convalescent patients who had SARS had a 1-way cross-reactivity with the 2 known HCoVs. Antigens of SARS-CoV and HCoV-OC43 were more cross-reactive than were those of SARS-CoV and HCoV-229E.
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