Information de reference pour ce titreAccession Number: | 00005245-200603010-00011.
|
Author: | Wang, Xiu-Li 1,2; Zhang, Hong-Mei 1; Li, De-Pei 1; Chen, Shao-Rui 1; Pan, Hui-Lin 1
|
Institution: | (1)Department of Anesthesiology, Department of Neural and Behavioral Sciences, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA (2)Department of Anesthesiology, The Third Hospital of HeBei Medical University, Shijiazhuang 050051, China
|
Title: | |
Source: | Journal of Physiology. 571(2):403-413, March 1, 2006.
|
Abstract: | Activation of spinal muscarinic acetylcholine receptors (mAChRs) inhibits nociception. However, the cellular mechanisms of this action are not fully known. In this study, we determined the role of mAChR subtypes in regulation of synaptic glycine release in the spinal cord. Whole-cell voltage-clamp recordings were performed on lamina II neurones in the rat spinal cord slices. The mAChR agonist oxotremorine-M significantly increased the frequency of glycinergic sIPSCs but not mIPSCs. Surprisingly, the effect of oxotremorine-M on sIPSCs was largely attenuated at a higher concentration. On the other hand, 1-10 [mu]M oxotremorine-M dose-dependently increased the frequency of sIPSCs in rats pretreated with intrathecal pertussis toxin. Furthermore, oxotremorine-M also dose-dependently increased the frequency of sIPSCs in the presence of himbacine (an M2/M4 mAChR antagonist) or AF-DX116 (an M2 mAChR antagonist). The M3 mAChR antagonist 4-DAMP abolished the stimulatory effect of oxotremorine-M on sIPSCs. Interestingly, the GABAB receptor antagonist CGP55845 potentiated the stimulatory effect of oxotremorine-M on sIPSCs. In the presence of CGP55845, both himbacine and AF-DX116 similarly reduced the potentiating effect of oxotremorine-M on sIPSCs. Collectively, these data suggest that the M3 subtype is present on the somatodendritic site of glycinergic neurones and is mainly responsible for muscarinic potentiation of glycinergic input to spinal dorsal horn neurones. Concurrent stimulation of mAChRs on adjacent GABAergic interneurones attenuates synaptic glycine release through presynaptic GABAB receptors on glycinergic interneurones. This study illustrates a complex dynamic interaction between GABAergic and glycinergic synapses in the spinal cord dorsal horn.
Copyright (C) 2006 Blackwell Publishing Ltd.
|
References: | Alfonzo MJ, De Becemberg IL, De Villaroel SS, De Herrera VN, Misle AJ & De Alfonzo RG (1998). Two opposite signal transducing mechanisms regulate a G-protein-coupled guanylyl cyclase. Arch Biochem Biophys 350, 19-25.
Blalock EM, Porter NM & Landfield PW (1999). Decreased G-protein-mediated regulation and shift in calcium channel types with age in hippocampal cultures. J Neurosci 19, 8674-8684.
Caulfield MP (1993). Muscarinic receptors - characterization, coupling and function. Pharmacol Ther 58, 319-379.
Caulfield MP & Birdsall NJ (1998). International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors. Pharmacol Rev 50, 279-290.
Chen YP, Chen SR & Pan HL (2005b). Effect of morphine on deep dorsal horn projection neurons depends on spinal GABAergic and glycinergic tone: implications for reduced opioid effect in neuropathic pain. J Pharmacol Exp Ther 315, 696-703.
Chen SR & Pan HL (2003). Spinal GABAB receptors mediate antinociceptive actions of cholinergic agents in normal and diabetic rats. Brain Res 965, 67-74.
Chen SR & Pan HL (2004). Activation of muscarinic receptors inhibits spinal dorsal horn projection neurons: role of GABAB receptors. Neuroscience 125, 141-148.
Chen SR, Wess J & Pan HL (2005aS). Functional activity of the M2 and M4 receptor subtypes in the spinal cord studied with muscarinic acetylcholine receptor knockout mice. J Pharmacol Exp Ther 313, 765-770.
Coelho JE, De Mendonca A & Ribeiro JA (2000). Presynaptic inhibitory receptors mediate the depression of synaptic transmission upon hypoxia in rat hippocampal slices. Brain Res 869, 158-165.
Cronin JN, Bradbury EJ & Lidierth M (2004). Laminar distribution of GABAA- and glycine-receptor mediated tonic inhibition in the dorsal horn of the rat lumbar spinal cord: effects of picrotoxin and strychnine on expression of Fos-like immunoreactivity. Pain 112, 156-163.
Doller D, Chackalamannil S, Czarniecki M, McQuade R & Ruperto V (1999). Design, synthesis, and structure-activity relationship studies of himbacine derived muscarinic receptor antagonists. Bioorg Med Chem Lett 9, 901-906.
Dorje F, Wess J, Lambrecht G, Tacke R, Mutschler E & Brann MR (1991). Antagonist binding profiles of five cloned human muscarinic receptor subtypes. J Pharmacol Exp Ther 256, 727-733.
Douglas CL, Baghdoyan HA & Lydic R (2001). M2 muscarinic autoreceptors modulate acetylcholine release in prefrontal cortex of C57BL/6J mouse. J Pharmacol Exp Ther 299, 960-966.
Duttaroy A, Gomeza J, Gan JW, Siddiqui N, Basile AS, Harman WD, Smith PL, Felder CC, Levey AI & Wess J (2002). Evaluation of muscarinic agonist-induced analgesia in muscarinic acetylcholine receptor knockout mice. Mol Pharmacol 62, 1084-1093.
Ehlert FJ (1996). The interaction of 4-DAMP mustard with subtypes of the muscarinic receptor. Life Sci 58, 1971-1978.
Felder CC (1995). Muscarinic acetylcholine receptors: signal transduction through multiple effectors. FASEB J 9, 619-625.
Fields TA & Casey PJ (1997). Signalling functions and biochemical properties of pertussis toxin-resistant G-proteins. Biochem J 321, 561-571.
Gomeza J, Shannon H, Kostenis E, Felder C, Zhang L, Brodkin J, Grinberg A, Sheng H & Wess J (1999). Pronounced pharmacologic deficits in M2 muscarinic acetylcholine receptor knockout mice. Proc Natl Acad Sci U S A 96, 1692-1697.
Hoglund AU & Baghdoyan HA (1997). M2, M3 and M4, but not M1, muscarinic receptor subtypes are present in rat spinal cord. J Pharmacol Exp Ther 281, 470-477.
Hood DD, Mallak KA, James RL, Tuttle R & Eisenach JC (1997). Enhancement of analgesia from systemic opioid in humans by spinal cholinesterase inhibition. J Pharmacol Exp Ther 282, 86-92.
Iwamoto ET & Marion L (1993). Characterization of the antinociception produced by intrathecally administered muscarinic agonists in rats. J Pharmacol Exp Ther 266, 329-338.
Jonas P, Bischofberger J & Sandkuhler J (1998). Corelease of two fast neurotransmitters at a central synapse. Science 281, 419-424.
Li DP, Chen SR, Pan YZ, Levey AI & Pan HL (2002). Role of presynaptic muscarinic and GABAB receptors in spinal glutamate release and cholinergic analgesia in rats. J Physiol 543, 807-818.
Miller JH, Aagaard PJ, Gibson VA & McKinney M (1992). Binding and functional selectivity of himbacine for cloned and neuronal muscarinic receptors. J Pharmacol Exp Ther 263, 663-667.
Murthy KS & Makhlouf GM (1997). Differential coupling of muscarinic M2 and M3 receptors to adenylyl cyclases V/VI in smooth muscle. Concurrent M2-mediated inhibition via G[alpha]i3 and M3-mediated stimulation via G[beta][gamma]q. J Biol Chem 272, 21317-21324.
Naguib M & Yaksh TL (1994). Antinociceptive effects of spinal cholinesterase inhibition and isobolographic analysis of the interaction with [mu] and [alpha]2 receptor systems. Anesthesiology 80, 1338-1348.
Pan YZ, Li DP & Pan HL (2002). Inhibition of glutamatergic synaptic input to spinal lamina II (o) neurons by presynaptic [alpha]2-adrenergic receptors. J Neurophysiol 87, 1938-1947.
Pan YZ & Pan HL (2004). Primary afferent stimulation differentially potentiates excitatory and inhibitory inputs to spinal lamina II outer and inner neurons. J Neurophysiol 91, 2413-2421.
Riley RC, Trafton JA, Chi SI & Basbaum AI (2001). Presynaptic regulation of spinal cord tachykinin signaling via GABAB but not GABAA receptor activation. Neuroscience 103, 725-737.
Scatton B, Dubois A, Javoy-Agid F & Camus A (1984). Autoradiographic localization of muscarinic cholinergic receptors at various segmental levels of the human spinal cord. Neurosci Lett 49, 239-245.
Todd AJ, Watt C, Spike RC & Sieghart W (1996). Colocalization of GABA, glycine, and their receptors at synapses in the rat spinal cord. J Neurosci 16, 974-982.
Villiger JW & Faull RL (1985). Muscarinic cholinergic receptors in the human spinal cord: differential localization of [3H]pirenzepine and [3H]quinuclidinylbenzilate binding sites. Brain Res 345, 196-199.
Wess J (1996). Molecular biology of muscarinic acetylcholine receptors. Crit Rev Neurobiol 10, 69-99.
Wong CS, Su YF, Chang KJ & Watkins WD (1992). Intrathecal pertussis toxin treatment attenuates opioid antinociception and reduces high-affinity state of opioid receptors. Anesthesiology 77, 691-699.
Yaksh TL (1989). Behavioral and autonomic correlates of the tactile evoked allodynia produced by spinal glycine inhibition: effects of modulatory receptor systems and excitatory amino acid antagonists. Pain 37, 111-123.
Yamamura HI, Wamsley JK, Deshmukh P & Roeske WR (1983). Differential light microscopic autoradiographic localization of muscarinic cholinergic receptors in the brainstem and spinal cord of the rat using [3H]pirenzepine. Eur J Pharmacol 91, 147-149.
Yigit M, Keipert C & Backus KH (2003). Muscarinic acetylcholine receptors potentiate the GABAergic transmission in the developing rat inferior colliculus. Neuropharmacology 45, 504-513.
Yoshimura M & Nishi S (1995). Primary afferent-evoked glycine- and GABA-mediated IPSPs in substantia gelatinosa neurones in the rat spinal cord in vitro. J Physiol 482, 29-38.
Yung KK & Lo YL (1997). Immunocytochemical localization of muscarinic M2 receptor in the rat spinal cord. Neurosci Lett 229, 81-84.
Zhang HM, Li DP, Chen SR & Pan HL (2005). M2, M3, and M4 receptor subtypes contribute to muscarinic potentiation of GABAergic inputs to spinal dorsal horn neurons. J Pharmacol Exp Ther 313, 697-704.
|
Language: | English.
|
Document Type: | NEUROSCIENCE.
|
Journal Subset: | Life Sciences.
|
ISSN: | 0022-3751
|
NLM Journal Code: | jqv, 0266262
|
Annotation(s) | |
|
|