Information de reference pour ce titreAccession Number: | 00007529-201311290-00054.
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Author: | Kong, Leopold 1,2,3; Giang, Erick 4; Nieusma, Travis 1; Kadam, Rameshwar U. 1; Cogburn, Kristin E. 1,4; Hua, Yuanzi 1; Dai, Xiaoping 1; Stanfield, Robyn L. 1,2,3; Burton, Dennis R. 2,3,4; Ward, Andrew B. 1,2,3*; Wilson, Ian A. 1,2,3,5*; Law, Mansun 4*
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Institution: | (1)Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.(2)International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA.(3)Scripps Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, The Scripps Research Institute, La Jolla, CA 92037, USA. (4)Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA. (5)Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
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Title: | |
Source: | Science. 342(6162):1090-1093, November 29, 2013.
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Abstract: | : Hepatitis C virus (HCV), a Hepacivirus, is a major cause of viral hepatitis, liver cirrhosis, and hepatocellular carcinoma. HCV envelope glycoproteins E1 and E2 mediate fusion and entry into host cells and are the primary targets of the humoral immune response. The crystal structure of the E2 core bound to broadly neutralizing antibody AR3C at 2.65 angstroms reveals a compact architecture composed of a central immunoglobulin-fold [beta] sandwich flanked by two additional protein layers. The CD81 receptor binding site was identified by electron microscopy and site-directed mutagenesis and overlaps with the AR3C epitope. The x-ray and electron microscopy E2 structures differ markedly from predictions of an extended, three-domain, class II fusion protein fold and therefore provide valuable information for HCV drug and vaccine design.
Copyright (C) 2013 by the American Association for the Advancement of Science
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Language: | English.
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Document Type: | REPORTS.
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Journal Subset: | Life Sciences. Physical Science & Engineering.
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ISSN: | 0036-8075
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NLM Journal Code: | 0404511, uj7
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DOI Number: | https://dx.doi.org/10.1126/scien...- ouverture dans une nouvelle fenêtre
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