The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells.
Stuparevic, Igor a,1,2; Becker, Emmanuelle a,2; Law, Michael J. b,2; Primig, Michael a,*,2
[Miscellaneous Article] FEBS Letters. 589(8):924-932, April 2, 2015.

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The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5'-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5'-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.

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* VTH1/VTH2 encode acetate-induced isoforms.

* The isoforms include an extended 5'-UTR.

* The long isoforms overlap antisense long non-coding RNAs.

* The VTH2 isoform is repressed in glucose by Rpd3/Ume6.

* Vth2 protein and VTH2 long isoform levels are negatively correlated.

(C) 2015 John Wiley & Sons, Ltd