A new approach to evaluate stability of amodiaquine and its metabolite in blood and plasma.
Blessborn, D. a; Neamin, G. a; Bergqvist, Y. a; Lindegardh, N. b,c,*
[Article]
Journal of Pharmaceutical and Biomedical Analysis.
41(1):207-212, April 2006.
(Format: HTML, PDF)
A stability study for amodiaquine (AQ) and desethylamodiaquine (AQm) in whole blood and plasma is reported. AQ, AQm and chloroquine (CQ) were simultaneously analysed and the ratios AQ/CQ and AQm/CQ were used to ensure correct interpretation of the stability results. CQ was stable in whole blood and plasma at all tested temperatures enabling it to be a stability marker in stability studies. Simultaneous analysis of compounds, of which at least one is already known to be stable, permits a within sample ratio to be used as a stability indicator. The new approach significantly reduced bias when compared to the traditional approach. AQ and AQm were stable in plasma at -86 [degrees]C and -20 [degrees]C for 35 days, at 4 [degrees]C for 14 days and at 22 [degrees]C for 1 day. AQ and AQm were stable in blood at -86 [degrees]C and 4 [degrees]C for 35 days, at -20 [degrees]C and 22 [degrees]C for 7 days and at 37 [degrees]C for 1 day.
(C) 2006Elsevier, Inc.