Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.
Yang, Neng; Higuchi, Osamu; Ohashi, Kazumasa; Nagata, Kyoko; Wada, Atsushi; Kangawa, Kenji; Nishida, Eisuke; Mizuno, Kensaku
[Letter]
Nature.
393(6687):809-812, June 25, 1998.
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Rac is a small GTPase of the Rho family that mediates stimulus-induced actin cytoskeletal reorganization to generate lamellipodia [1-5]. Little is known about the signalling pathways that link Rac activation to changes in actin filament dynamics. Cofilin is known to be a potent regulator of actin filament dynamics [6-10], and its ability to bind and depolymerize actin is abolished by phosphorylation of serine residue at 3 [11,12]; however, the kinases responsible for this phosphorylation have not been identified. Here we show that LIM-kinase 1 (LIMK-1), a serine/threonine kinase containing LIM and PDZ domains [13-16], phosphorylates cofilin at Ser 3, both in vitro and in vivo. When expressed in cultured cells, LIMK-1 induces actin reorganization and reverses cofilin-induced actin depolymerization. Expression of an inactive form of LIMK-1 suppresses lamellipodium formation induced by Rac or insulin. Furthermore, insulin and an active form of Rac increase the activity of LIMK-1. Taken together, our results indicate that LIMK-1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphorylating cofilin.
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