Information de reference pour ce titreAccession Number: | 00005797-200808040-00003.
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Author: | Coros, Abbie 1,+; Callahan, Brian 1,+; Battaglioli, Eric 1; Derbyshire, Keith M. 1,2,*
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Institution: | (1)Division of Infectious Disease, Wadsworth Center, New York State Department of Health, NY, USA. (2)Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, NY 12201-2002, USA.
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Title: | |
Source: | Molecular Microbiology. 69(4):794-808, August 2008.
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Abstract: | Summary: Conjugal DNA transfer in Mycobacterium smegmatis occurs by a mechanism distinct from plasmid-mediated DNA transfer. Previously, we had shown that the secretory apparatus, ESX-1, negatively regulated DNA transfer from the donor strain; ESX-1 donor mutants are hyper-conjugative. Here, we describe a genome-wide transposon mutagenesis screen to isolate recipient mutants. Surprisingly, we find that a majority of insertions map within the esx-1 locus, which encodes the secretory apparatus. Thus, in contrast to its role in donor function, ESX-1 is essential for recipient function; recipient ESX-1 mutants are hypo-conjugative. In addition to esx-1 genes, our screen identifies novel non-esx-1 loci in the M. smegmatis genome that are required for both DNA transfer and ESX-1 activity. DNA transfer therefore provides a simple molecular genetic assay to characterize ESX-1, which, in Mycobacterium tuberculosis, is necessary for full virulence. These findings reinforce the functional intertwining of DNA transfer and ESX-1 secretion, first described in the M. smegmatis donor. Moreover, our observation that ESX-1 has such diametrically opposed effects on transfer in the donor and recipient, forces us to consider how proteins secreted by the ESX-1 apparatus can function so as to modulate two seemingly disparate processes, M. smegmatis DNA transfer and M. tuberculosis virulence.
Copyright (C) 2008 Blackwell Publishing Ltd.
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Language: | English.
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Document Type: | Research Article.
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Journal Subset: | Life Sciences.
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ISSN: | 0950-382X
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NLM Journal Code: | mom, 8712028
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