Information de reference pour ce titreAccession Number: | 00005797-200701010-00021.
|
Author: | Stinnett, Suzanne M. 1,+; Espeso, Eduardo A. 2,+; Cobeno, Laura 2; Araujo-Bazan, Lidia 2; Calvo, Ana M. 1,*
|
Institution: | (1)Northern Illinois University, Biological Sciences, 1425 W. Lincoln Hwy Montgomery Hall, Dekalb, IL 60115, USA. (2)Centro de Investigaciones Biologicas (C.S.I.C.), Microbiologia Molecular, Ramiro de Maeztu, 9. 28040 Madrid, Spain.
|
Title: | |
Source: | Molecular Microbiology. 63(1):242-255, January 2007.
|
Abstract: | Summary: The veA gene is a light-dependent regulator governing development and secondary metabolism in Aspergillus nidulans. We have identified a putative bipartite nuclear localization signal (NLS) motif in the A. nidulans VeA amino acid sequence and demonstrated its functionality when expressed in yeast. Furthermore, migration of VeA to the nucleus was dependent on the importin [alpha]. This bipartite NLS is also functional when VeA is expressed in A. nidulans. Interestingly, we found that VeA migration to the nucleus is light-dependent. While in the dark VeA is located mainly in the nuclei, under light VeA is found abundantly in the cytoplasm. The VeA1 mutant protein (lacking the first 36 amino acids at the N-terminus) was found predominantly in the cytoplasm independent of illumination. This indicates that the truncated bipartite NLS in VeA1 is not functional and fails to respond to light. These results might explain the lack of the morphological light-dependent response in strains carrying the veA1 allele. We also evaluated the effect of light on production of the mycotoxin sterigmatocystin in a veA wild-type and the veA1 mutant strains and found that the highest amount of toxin was produced by the veA+ strain growing in the dark, condition favouring accumulation of VeA in the nucleus.
Copyright (C) 2007 Blackwell Publishing Ltd.
|
References: | Adams, T.H., and Yu, J.H. (1998) Coordinate control of secondary metabolite production and asexual sporulation in Aspergillus nidulans. Curr Opin Microbiol 1: 674-677.
Alexopoulos, C.J. (1962) Introductory Mycology. New York: John Wiley and Sons.
Alexopoulos, C.J., Mims, C.W., and Blackwell, M. (1996) Introductory Mycology, 4th edn. New York: John Wiley and Sons.
Bernreiter, A., Ramon, A., Fernandez-Martinez, J., Berger, H., Araujo-Bazan, L., Espeso, E.A., et al. (2006) Nuclear export of the transcription factor NirA is a regulatory checkpoint for nitrate induction in Aspergillus nidulans. Mol Cell Biol (in press).
Blumenstein, A., Vienken, K., Tasler, R., Purschwitz, J., Veith, D., Frankenberg-Dinkel, N., and Fischer, R. (2005) The Aspergillus nidulans phytochrome FphA represses sexual development in red light. Curr Biol 15: 1833-1838.
Braus, G.H., Krappmann, S., and Eckert, S.E. (2002) Sexual development in ascomycetes: fruit body formation of Aspergillus nidulans. In Molecular Biology of Fungal Development. Osiewacz, H.D. (ed.). New York: Marcel Decker, pp. 215-244.
Calvo, A.M., Gardner, H.W., and Keller, N.P. (2001) Genetic connection between fatty acid metabolism and sporulation in Aspergillus nidulans. J Biol Chem 276: 25766-25774.
Calvo, A.M., Wilson, R.A., Bok, J.W., and Keller, N.P. (2002) Relationship between secondary metabolism and fungal development. Microbiol Mol Biol Rev 66: 447-459.
Calvo, A.M., Bok, J., Brooks, W., and Keller, N.P. (2004) veA is required for toxin and sclerotial production in Aspergillus parasiticus. Appl Environ Microbiol 70: 4733-4739.
Champe, S.P., Nagle, D.L., and Yager, L.N. (1994) Sexual sporulation. Prog Ind Microbiol 29: 429-454.
Clutterbuck, A.J. (1977) The genetics of conidiation in Aspergillus nidulans. In Genetics and Physiology of Aspergillus. Smith, J.E., and Pateman, J.A. (eds). London: Academic Press, pp. 305-317.
Duran, R.M., Cary, J.W., and Calvo, A.M. (2006) Production of cyclopiazonic acid, aflatrem, and aflatoxin by Aspergillus flavus is regulated by veA, a gene necessary for sclerotial formation. Appl Microbiol Biotechnol (Epub).
Fernandez-Martinez, J., Brown, C.V., Diez, E., Tilburn, J., Arst, H.N., Jr, Penalva, M.A., and Espeso, E.A. (2003) Overlap of nuclear localisation signal and specific DNA-binding residues within the zinc finger domain of PacC. J Mol Biol 334: 667-684.
Fontes, M.R., Teh, T., and Kobe, B. (2000) Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha. J Mol Biol 297: 1183-1194.
Fontes, M.R., Teh, T., Jans, D., Brinkworth, R.I., and Kobe, B. (2003a) Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha. J Biol Chem 278: 27981-27987.
Fontes, M.R., Teh, T., Toth, G., John, A., Pavo, I., Jans, D.A., and Kobe, B. (2003b) Role of flanking sequences and phosphorylation in the recognition of the simian-virus-40 large T-antigen nuclear localization sequences by importin-alpha. Biochem J 375: 339-349.
Froehlich, A.C., Liu, Y., Loros, J.J., and Dunlap, J.C. (2002) White collar-1, a circadian blue light photoreceptor, binding to the frequent promoter. Science 297: 815-819.
Han, S., and Adams, T.H. (2001) Complex control of the developmental regulatory locus brlA in Aspergillus nidulans. Mol Genet Genomics 266: 260-270.
Han, S., Navarro, J., Greve, R.A., and Adams, T.H. (1993) Translational repression of brlA expression prevents premature development in Aspergillus. EMBO J 12: 2449-2457.
He, Q., Cheng, P., Yang, Y., Wang, L., Gardner, K.H., and Liu, Y. (2002) White collar-1, a DNA binding transcription factor and a light sensor. Science 219: 840-843.
Hicks, J.K., Yu, J.H., Keller, N.P., and Adams, T.H. (1997) Aspergillus sporulation and mycotoxin production both require inactivation of the FadA G alpha protein-dependent signaling pathway. EMBO J 16: 4916-4923.
Idnurm, A., and Heitman, J. (2005) Photosensing fungi: phytochrome in the spotlight. Curr Biol 15: 829-832.
Kafer, E. (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 19: 33-131.
Kato, N., Brooks, W., and Calvo, A.M. (2003) The expression of sterigmatocystin and penicillin genes in Aspergillus nidulans is controlled by veA, a gene required for sexual development. Eukaryot Cell 2: 1178-1186.
Kim, H., Han, K., Kim, K., Han, D., Jahng, K., and Chae, K. (2002) The veA gene activates sexual development in Aspergillus nidulans. Fungal Genet Biol 37: 72-80.
Kutay, U., and Guttinger, S. (2005) Leucine-rich nuclear-export signals: born to be weak. Trends Cell Biol 15: 121-124.
Lee, B.N., and Adams, T.H. (1994) Overexpression of flbA, an early regulator of Aspergillus asexual sporulation, leads to activation of brlA and premature initiation of development. Mol Microbiol 14: 323-334.
Liu, Y., He, Q., and Cheng, P. (2003) Photoreception in Neurospora: a tale of two White Collar proteins. Cell Mol Life Sci 60: 2131-2138.
McCurdy, D.W., and Pratt, L.H. (1986) Immunogold electron microscopy of phytochrome in Avena: identification of intracellular sites responsible for phytochrome sequestering and enhanced pelletability. J Cell Biol 103: 2541-2550.
Miller, B.L., Miller, K.Y., and Timberlake, W.E. (1985) Direct and indirect gene replacements in Aspergillus nidulans. Mol Cell Biol 5: 1714-1721.
Mooney, J.L., and Yager, L.N. (1990) Light is required for conidiation in Aspergillus nidulans. Genes Dev 4: 1473-1482.
Mooney, J.L., Hassett, D.E., and Yager, L.N. (1990) Genetic analysis of suppressors of the veA1 mutation in Aspergillus nidulans. Genetics 126: 869-874.
Nair, R., Carter, P., and Rost, B. (2003) NLSdb: database of nuclear localization signals. Nucleic Acids Res 31: 397-399.
Penalva, M.A., and Arst, H.N., Jr (2004) Recent advances in the characterization of ambient pH regulation of gene expression in filamentous fungi and yeasts. Annu Rev Microbiol 58: 425-451.
Shimizu, K., and Keller, N.P. (2001) Genetic involvement of a cAMP-dependent protein kinase in a G protein signaling pathway regulating morphological and chemical transitions in Aspergillus nidulans. Genetics 157: 591-600.
Sikorski, R.S., and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
Skromne, I., Sanchez, O., and Aguirre, J. (1995) Starvation stress modulates the expression of the Aspergillus nidulans brlA regulatory gene. Microbiology 141: 21-28.
Toews, M.W., Warmbold, J., Konzack, S., Rischitor, P., Veith, D., Vienken, K., et al. (2004) Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (GATEWAY). Curr Genet 45: 383-389.
Tsitsigiannis, D.I., Zarnowski, R., and Keller, N.P. (2004a) The lipid body protein, PpoA, coordinates sexual and asexual sporulation in Aspergillus nidulans. J Biol Chem 279: 11344-11353.
Tsitsigiannis, D.I., Kowieski, T.M., Zarnowski, R., and Keller, N.P. (2004b) Endogenous lipogenic regulators of spore balance in Aspergillus nidulans. Eukaryot Cell 3: 1398-1411.
Tsitsigiannis, D.I., Kowieski, T.M., Zarnowski, R., andKeller, N.P. (2005) Three putative oxylipin biosynthetic genes integrate sexual and asexual development in Aspergillus nidulans. Microbiology 151: 1809-1821.
Vincent, O., Rainbow, L., Tilburn, J., Arst, H.N., and Penalva, M.A. (2002) YPXL/I is a protein interaction motif recognized by Aspergillus PalA and its human homologue AIP1/Alix. Mol Cell Biol 23: 1647-1655.
Yager, L.N. (1992) Early developmental events during asexual and sexual sporulation in Aspergillus nidulans. Biotechnology 23: 19-41.
Yager, L.N., Lee, H., Nagle, D.L., and Zimmerman, J.E. (1998) Analysis of fluG mutations that affect light-dependent conidiation in Aspergillus nidulans. Genetics 149: 1777-1786.
Yang, L., Ukil, L., Osmani, A., Nahm, F., Davies, J., De Souza, C.P.C., et al. (2004) Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans. Eukaryot Cell 3: 1359-1362.
|
Language: | English.
|
Document Type: | Research Article.
|
Journal Subset: | Life Sciences.
|
ISSN: | 0950-382X
|
NLM Journal Code: | mom, 8712028
|
Annotation(s) | |
|
|