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THE effects of neurotrophins on intracellular Ca2 levels in rat hippocampal neurones were studied in vitro using fura-2 fluorescence microscopy. BDNF and NT-3, but not NGF, rapidly increased cytoplasmic Ca2 concentrations in these neurones ten-fold to reach 1 [mu]M. Moreover in some of the neurones both BDNF and NT-3 elicited Ca2 responses, indicative of the presence of functional receptors for these neurotrophins in the same cell. In these cultures approximately 80% of the hippocampal neurones were stained with antibodies against full-length Trk B. The expression of functional Trk B was also confirmed by RNA analysis. These results demonstrate the presence of functional receptors for BDNF and NT-3 in hippocampal neurones.

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