Comparison of aerobic denitrification under high oxygen atmosphere by Thiosphaera pantotropha ATCC 35512 and Pseudomonas stutzeri SU2 newly isolated from the activated sludge of a piggery wastewater treatment system.
Su, J.-J.; Liu, B.-Y.; Liu, C.-Y.
[Article] Journal of Applied Microbiology. 90(3):457-462, March 2001.

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Aims: This study compares the ability of Thiosphaera pantotropha ATCC 35512 and the newly isolated Pseudomonas stutzeri SU2 to perform aerobic denitrification.

Methods and Results: Nitrate-supplemented basal medium in airtight crimp-sealed serum bottles containing an atmosphere of 92% oxygen was inoculated with Ps. stutzeri SU2 or T. pantotropha and incubated at 30 [degrees]C. During the 92-h incubation period, aerobic denitrification by Ps. stutzeri SU2 (NO3- - N removal 99[middle dot]24%) was more efficient than that by T. pantotropha (NO3- - N removal 27[middle dot]29%).

Conclusions: Pseudomonas stutzeri SU2, which was isolated from the activated sludge of a sequencing batch reactor treating piggery wastewater, rapidly reduced the nitrate to nitrogen gas without nitrite accumulation. The nitrate removal rate of SU2 was 0[middle dot]032 mmol NO3- - N g cell-1 h-1 after 44 h incubation.

Significance and Impact of the Study: Pseudomonas stutzeri SU2 can be used in a full-scale sequencing batch system for efficient in situ aerobic nitrate removal from piggery wastewater.

(C) 2001 The Society for Applied Microbiology