Information de reference pour ce titreAccession Number: | 00042754-200412000-00013.
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Author: | Fields, Patrick E. 1,4; Lee, Gap Ryol 1,4; Kim, Sean T. 1,4; Bartsevich, Victor V. 3; Flavell, Richard A. *,1,2
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Institution: | (1)Section of Immunobiology and Yale University School of Medicine, New Haven, CT 06520 USA (2)The Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520 USA (3)Sangamo BioSciences, Inc., Richmond, CA 94804 USA
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Title: | Th2-Specific Chromatin Remodeling and Enhancer Activity in the Th2 Cytokine Locus Control Region.[Article]
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Source: | Immunity. 21(6):865-876, December 2004.
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Abstract: | Summary: We recently identified a 3' region of the rad50 gene possessing strong enhancer activity as well as activity consistent with function as a locus control region (LCR) for the flanking Th2 cytokine genes. In this study, we identify several functional elements within this region by examining chromatin changes as well as activity in transgenic mice. We find within this region four DNase I hypersensitive clusters, three of which are highly conserved and predominantly expressed in Th2 cells. Histone acetylation of this region is elevated in Th2 cells. Further, one of the hypersensitive sites (RHS7) is rapidly demethylated in Th2, but not Th1, cells. In transgenic mice, these hypersensitive sites impart strong, Th2-specific enhancer activity as well as copy number-dependent expression of the reporter gene, recapitulating LCR function. We postulate that these sites function alone or in combination with other regulatory elements to coordinate gene expression in the Th2 cytokine locus.
(C) 2004Elsevier, Inc.
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References: | Agarwal, S., Rao, A. (1998). Modulation of chromatin structure regulates cytokine gene expression during T cell differentiation. Immunity 9, 765-775.
Aizawa, A., Yoneyama, T., Kazahari, K., Ono, M. (1995). DNase I-hypersensitive sites in the chromatin of rat growth hormone gene locus and enhancer activity of regions with these sites. Nucleic Acids Res 23, 2236-2244.
Avni, O., Lee, D., Macian, F., Szabo, S.J., Glimcher, L.H., Rao, A. (2002). T(H) cell differentiation is accompanied by dynamic changes in histone acetylation of cytokine genes. Nat. Immunol 3, 643-651.
Bell, A.C., Felsenfeld, G. (2000). Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene. Nature >405, 482-485.
Bird, J.J., Brown, D.R., Mullen, A.C., Moskowitz, N.H., Mahowald, M.A., Sider, J.R., Gajewski, T.F., Wang, C.R., Reiner, S.L. (1998). Helper T cell differentiation is controlled by the cell cycle. Immunity 9, 229-237.
Bungert, J., Dave, U., Lim, K.C., Lieuw, K.H., Shavit, J.A., Liu, Q., Engel, J.D. (1995). Synergistic regulation of human beta-globin gene switching by locus control region elements HS3 and HS4. Genes Dev 9, 3083-3096.
Cousins, D.J., Lee, T.H., Staynov, D.Z. (2002). Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J. Immunol 169, 2498-2506.
Curtin, P.T., Liu, D.P., Liu, W., Chang, J.C., Kan, Y.W. (1989). Human beta-globin gene expression in transgenic mice is enhanced by a distant DNase I hypersensitive site. Proc. Natl. Acad. Sci. USA 86, 7082-7086.
Elefant, F., Su, Y., Liebhaber, S.A., Cooke, N.E. (2000). Patterns of histone acetylation suggest dual pathways for gene activation by a bifunctional locus control region. EMBO J 19, 6814-6822.
Fields, P.E., Kim, S.T., Flavell, R.A. (2002). Cutting edge: changes in histone acetylation at the IL-4 and IFN-gamma loci accompany Th1/Th2 differentiation. J. Immunol 169, 647-650.
Fraser, P., Hurst, J., Collis, P., Grosveld, F. (1990). DNaseI hypersensitive sites 1, 2 and 3 of the human beta-globin dominant control region direct position-independent expression. Nucleic Acids Res 18, 3503-3508.
Grosveld, F., van Assendelft, G.B., Greaves, D.R., Kollias, G. (1987). Position-independent, high-level expression of the human beta-globin gene in transgenic mice. Cell 51, 975-985.
Jackson, J.D., Petrykowska, H., Philipsen, S., Miller, W., Hardison, R. (1996). Role of DNA sequences outside the cores of DNase hypersensitive sites (HSs) in functions of the beta-globin locus control region. Domain opening and synergism between HS 2(and HS3. J. Biol. Chem. 271). 11871-11878.
Kelly, B.L., Locksley, R.M. (2000). Coordinate regulation of the IL-4, IL-13, and IL-5 cytokine cluster in Th2 clones revealed by allelic expression patterns. J. Immunol 165, 2982-2986.
Lee, D.U., Agarwal, S., Rao, A. (2002). Th2 lineage commitment and efficient IL-4 production involves extended demethylation of the IL-4 gene. Immunity 16, 649-660.
Lee, G.R., Fields, P.E., Flavell, R.A. (2001). Regulation of IL-4 gene expression by distal regulatory elements and GATA-3 at the chromatin level. Immunity 14, 447-459.
Lee, G.R., Fields, P.E., Griffin, T.J., Flavell, R.A. (2003). Regulation of the Th2 cytokine locus by a locus control region. Immunity 19, 145-153.
Li, Q., Stamatoyannopoulos, G. (1994). Hypersensitive site 5 of the human beta locus control region functions as a chromatin insulator. Blood 84, 1399-1401.
Li, Q., Peterson, K.R., Fang, X., Stamatoyannopoulos, G. (2002). Locus control regions. Blood 100, 3077-3086.
Litt, M.D., Simpson, M., Recillas-Targa, F., Prioleau, M.N., Felsenfeld, G. (2001). Transitions in histone acetylation reveal boundaries of three separately regulated neighboring loci. EMBO J 20, 2224-2235.
Loots, G.G., Locksley, R.M., Blankespoor, C.M., Wang, Z.E., Miller, W., Rubin, E.M., Frazer, K.A. (2000). Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons. Science 288, 136-140.
Milot, E., Strouboulis, J., Trimborn, T., Wijgerde, M., de Boer, E., Langeveld, A., Tan-Un, K., Vergeer, W., Yannoutsos, N., Grosveld, F., Fraser, P. (1996). Heterochromatin effects on the frequency and duration of LCR-mediated gene transcription. Cell 87, 105-114.
Mohrs, M., Blankespoor, C.M., Wang, Z.E., Loots, G.G., Afzal, V., Hadeiba, H., Shinkai, K., Rubin, E.M., Locksley, R.M. (2001). Deletion of a coordinate regulator of type 2 cytokine expression in mice. Nat. Immunol 2, 842-847.
Molete, J.M., Petrykowska, H., Bouhassira, E.E., Feng, Y.Q., Miller, W., Hardison, R.C. (2001). Sequences flanking hypersensitive sites of the beta-globin locus control region are required for synergistic enhancement. Mol. Cell. Biol 21, 2969-2980.
Ortiz, B.D., Cado, D., Winoto, A. (1999). A new element within the T-cell receptor alpha locus required for tissue-specific locus control region activity. Mol. Cell. Biol 19, 1901-1909.
Ryan, T.M., Behringer, R.R., Martin, N.C., Townes, T.M., Palmiter, R.D., Brinster, R.L. (1989). A single erythroid-specific DNase I super-hypersensitive site activates high levels of human beta-globin gene expression in transgenic mice. Genes Dev 3, 314-323.
Santoso, B., Ortiz, B.D., Winoto, A. (2000). Control of organ-specific demethylation by an element of the T-cell receptor-alpha locus control region. J. Biol. Chem 275, 1952-1958.
Sawado, T., Halow, J., Bender, M.A., Groudine, M. (2003). The beta-globin locus control region (LCR) functions primarily by enhancing the transition from transcription initiation to elongation. Genes Dev 17, 1009-1018.
Schubeler, D., Francastel, C., Cimbora, D.M., Reik, A., Martin, D.I., Groudine, M. (2000). Nuclear localization and histone acetylation: a pathway for chromatin opening and transcriptional activation of the human beta-globin locus. Genes Dev 14, 940-950.
Schubeler, D., Groudine, M., Bender, M.A. (2001). The murine beta-globin locus control region regulates the rate of transcription but not the hyperacetylation of histones at the active genes. Proc. Natl. Acad. Sci. USA 98, 11432-11437.
Simon, I., Tenzen, T., Mostoslavsky, R., Fibach, E., Lande, L., Milot, E., Gribnau, J., Grosveld, F., Fraser, P., Cedar, H. (2001). Developmental regulation of DNA replication timing at the human beta globin locus. EMBO J 20, 6150-6157.
Smale, S.T., Fisher, A.G. (2002). Chromatin structure and gene regulation in the immune system. Annu. Rev. Immunol 20, 427-462.
Spilianakis, C.G., Flavell, R.A. (2004). Long-range intrachromosomal interactions in the T helper type 2 cytokine locus. Nat. Immunol 5, 1017-1027.
Su, Y., Liebhaber, S.A., Cooke, N.E. (2000). The human growth hormone gene cluster locus control region supports position-independent pituitary- and placenta-specific expression in the transgenic mouse. J. Biol. Chem 275, 7902-7909.
Takemoto, N., Koyano-Nakagawa, N., Yokota, T., Arai, N., Miyatake, S., Arai, K. (1998). Th2-specific DNase I-hypersensitive sites in the murine IL-13 and IL-4 intergenic region. Int. Immunol 10, 1981-1985.
Waalwijk, C., Flavell, R.A. (1978). MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites. Nucleic Acids Res 5, 3231-3236.
Yamashita, M., Ukai-Tadenuma, M., Kimura, M., Omori, M., Inami, M., Taniguchi, M., Nakayama, T. (2002). Identification of a conserved GATA3 response element upstream proximal from the interleukin-13 gene locus. J. Biol. Chem 277, 42399-42408.
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Language: | English.
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Document Type: | Article.
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Journal Subset: | Clinical Medicine. Life & Biomedical Sciences.
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ISSN: | 1074-7613
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NLM Journal Code: | 9432918, ccf
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DOI Number: | https://dx.doi.org/10.1016/j.imm...- ouverture dans une nouvelle fenêtre
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