Information de reference pour ce titreAccession Number: | 00008572-199808030-00015.
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Author: | Boevink, Petra 1; Oparka, Karl 1; Cruz, Simon Santa 1; Martin, Barry 2; Betteridge, Alan 2; Hawes, Chris 2,*
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Institution: | (1)Unit of Cell Biology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK, and (2)Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, UK
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Title: | Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network+.[Miscellaneous Article]
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Source: | Plant Journal. 15(3):441-447, August 1998.
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Abstract: | Summary: We have visualized the relationship between the endoplasmic reticulum (ER) and Golgi in leaf cells of Nicotiana clevelandii by expression of two Golgi proteins fused to green fluorescent protein(GFP). A fusion of the trans-membrane domain (signal anchor sequence) of a rat sialyl transferase to GFP was targeted to the Golgi stacks. A second construct that expressed the Arabidopsis H/KDEL receptor homologue aERD2, fused to GFP, was targeted to both the Golgi apparatus and ER, allowing the relationship between these two organelles to be studied in living cells for the first time. The Golgi stacks were shown to move rapidly and extensively along the polygonal cortical ER network of leaf epidermal cells, without departing from the ER tubules. Co-localization of F-actin in the GFP-expressing cells revealed an underlying actin cytoskeleton that matched precisely the architecture of the ER network, while treatment of cells with the inhibitors cytochalasin D and N-ethylmaleimide revealed the dependency of Golgi movement on actin cables. These observations suggest that the leaf Golgi complex functions as a motile system of actin-directed stacks whose function is to pick up products from a relatively stationary ER system. Also, we demonstrate for the first time in vivo brefeldin A-induced retrograde transport of Golgi membrane protein to the ER.
(C) 1998 Blackwell Science Ltd.
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Language: | English.
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Document Type: | Short Communication.
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Journal Subset: | Life Sciences.
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ISSN: | 0960-7412
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NLM Journal Code: | bru, 9207397
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