Differential nociceptive responses in mice lacking the [alpha]1B subunit of N-type Ca2+ channels .
Hatakeyama, Shinji 1,2, CA; Wakamori, Minoru 3; Ino, Mitsuhiro 2; Miyamoto, Norimasa 2; Takahashi, Eiki 2; Yoshinaga, Takashi 2; Sawada, Kohei 2; Imoto, Keiji 3; Tanaka, Isao 2; Yoshizawa, Toshihiro 1; Nishizawa, Yukio 2; Mori, Yasuo 3,4; Niidome, Tetsuhiro 2; Shoji, Shin'ich 1
12(11):2423-2427, August 8, 2001.
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The role of N-type Ca2 channels in nociceptive transmission was examined in genetically engineered mice lacking the [alpha]1B subunit of N-type channels and in their heterozygote and wild-type littermates. In [alpha]1B-deficient mice, N-type channel activities in dorsal root ganglion neurons and spinal synaptoneurosomes were eliminated without compensation by other types of voltage-dependent Ca2 channels. The [alpha]1B-deficient mice showed a diminution in the phase 2 nociceptive responses more extensively than in the phase 1 nociceptive responses of the formalin test. The [alpha]1B-deficient mice exhibited significantly increased thermal nociceptive thresholds in the hot plate test, but failed to increase mechanical nociceptive thresholds in the tail pinch test. These results suggest a crucial role of N-type channels in nociceptive transmission, especially for persistent pain like phase 2 of the formalin test and for nociception induced by thermal stimuli.
(C) 2001 Lippincott Williams & Wilkins, Inc.