A Combined Assay of hTERT and E6 Oncoprotein to Identify Virus-Infected Keratinocytes With Higher Telomerase Activity in Human Papillomaviruses 16 and 18-Related Bowenoid Papulosis.
Li, Dongsheng MD; Dong, Bilin PhD; Hu, Zhimin PhD; Chen, Liuqing MD; Zeng, Xianyu MD; Chen, Jinbo MD; Duan, Yiqun MD
American Journal of Dermatopathology.
34(8):813-817, December 2012.
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In the present study, we aim to evaluate the application potential of a combined assay of human telomerase reverse transcriptase (hTERT) and E6 oncoprotein in screening the virus-infected keratinocytes with higher telomerase activity in human papillomaviruses (HPV) 16- and 18-related bowenoid papulosis (BP). HPV16/18 DNA in BP (n = 123) was identified by in situ hybridization, the expression of hTERT and E6 in HPV16/18-related BP (n = 68) was determined by immunohistochemistry. We demonstrated that the expression of hTERT correlated well with that of E6 oncoprotein in HPV16/18-related BP lesions (Spearman rho = 0.868, P < 0.01). Furthermore, the majority of keratinocytes with positive nuclear staining for hTERT or E6 in the consecutive sections of each HPV16/18-related BP lesion showed nuclear paleomorphism or nuclear mitosis. In conclusion, we suggested that a combined assay of hTERT and E6 oncoprotein can be used to screen the HPV-infected keratinocytes with higher telomerase activity in HPV16-related and HPV18-related BP lesions.
(C) 2012 Lippincott Williams & Wilkins, Inc.