Information de reference pour ce titreAccession Number: | 00001758-199735060-00023.
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Author: | Kang, Tae-Jin; Fawley, Marvin W. *
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Institution: | Department of Botany, North Dakota State University, P.O. Box 5517 Fargo, ND 58105-5517, USA
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Title: | Variable (CA/GT)n simple sequence repeat DNA in the alga Chlamydomonas.[Miscellaneous]
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Source: | Plant Molecular Biology. 35(6):943-948, December 1997.
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Abstract: | A partial genomic DNA library of Chlamydomonas reinhardtii was screened with an (AC)11 probe for the presence of (CA/GT)n simple sequence repeats (SSRs). Based on the frequency of these repeats in the partial genomic library, we estimate that (CA/GT)n repeats occur at a rate of about one every 17.7 kb in the C. reinhardtii genome. Ten positive clones were sequenced and four polymerase chain reaction (PCR) primer sets flanking (CA/GT)n sequences were constructed for four loci. The PCR was used to specifically amplify these regions from multiple isolates of C. reinhardtii. All four loci were highly polymorphic in the C. reinhardtii isolates. A simple Mendelian inheritance pattern was found for all four loci, which showed 2:2 segregation in the tetrads resulting from a cross between C. reinhardtii and C. smithii. Our results suggest that these simple sequence repeat DNA loci will be useful for identity testing, population studies, linkage analysis, and genome mapping in Chlamydomonas.
(C)1997 Kluwer Academic Publishers
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Author Keywords: | Chlamydomonas; Chlorophyta; microsatellite; polymorphism; simple sequence repeat DNA.
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Language: | English.
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Document Type: | Short communication: PDF Only.
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Journal Subset: | Life Sciences.
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ISSN: | 0167-4412
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NLM Journal Code: | a6o, 9106343
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Annotation(s) | |