Information de reference pour ce titreAccession Number: | 00125945-200712000-00009.
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Author: | Zhang, Rui 1,+; Weinbauer, Markus G. 2,+; Qian, Pei-Yuan 1,*
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Institution: | (1)Department of Biology and Coastal Marine Laboratory, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. (2)Microbial Ecology and Biogeochemistry Group, Universite Pierre et Marie Curie-Paris 6, Laboratoire d'Oceanographie de Villefranche, 06230 Villefranche-sur-Mer, France; CNRS, Laboratoire d'Oceanographie de Villefranche, 06230 Villefranche-sur-Mer, France.
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Title: | |
Source: | Environmental Microbiology. 9(12):3008-3018, December 2007.
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Abstract: | Summary: To gain a better understanding of the interactions among bacteria, viruses and flagellates in coastal marine ecosystems, we investigated the effect of viral lysis and protistan bacterivory on bacterial abundance, production and diversity [determined by 16S rRNA gene polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE)] in three coastal marine sites with different nutrient supplies in Hong Kong. Six experiments were set up using filtration and dilution methods to develop virus, flagellate and virus+flagellate treatments for natural bacterial populations. All three predation treatments had significant repressing effects on bacterial abundance. Bacterial production was significantly repressed by flagellates and both predators (flagellates and viruses). Bacterial apparent species richness (indicated as the number of DGGE bands) was always significantly higher in the presence of viruses, flagellates and both predators than in the predator-free control. Cluster analysis of the DGGE patterns showed that the effects of viruses and flagellates on bacterial community structure were relatively stochastic while the co-effects of predators caused consistent trends (DGGE always showed the most similar patterns when compared with those of in situ environments) and substantially increased the apparent richness. Overall, we found strong evidence that viral lysis and protist bacterivory act additively to reduce bacterial production and to sustain diversity. This first systematic attempt to study the interactive effects of viruses and flagellates on the diversity and production of bacterial communities in coastal waters suggests that a tight control of bacterioplankton dominants results in relatively stable bacterioplankton communities.
Copyright (C) 2007 Blackwell Publishing Ltd.
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References: | Bell, T., Ager, D., Song, J.-I., Newman, J.A., Thompson, I.P., Lilley, A.K., and van der Gast, C.J. (2005) Larger islands house more bacterial taxa. Science 308: 1884.
Bouvier, T., and del Giorgio, P.A. (2007) Key role of selective viral-induced mortality in determining marine bacterial community composition. Environ Microbiol 9: 287-297.
Brussaard, C.P.D. (2004) Optimization of procedures for counting viruses by flow cytometry. Appl Environ Microbiol 70: 1506-1513.
Buckling, A., Kassen, R., Bell, G., and Rainey, P.B. (2000) Disturbance and diversity in experimental microcosms. Nature 408: 961-964.
Curtis, T.P., Sloan, W.T., and Scannell, J.W. (2002) Estimating prokaryotic diversity and its limits. Proc Natl Acad Sci USA 99: 10494-10499.
DeLong, E.F. (1992) Archaea in coastal marine environments. Proc Natl Acad Sci USA 89: 5685-5689.
Ducklow, H.W., and Carlson, C.A. (1992) Oceanic bacterial production. In Advance in Microbial Ecology, Vol. 12. Marshall, K.C. (ed). New York, USA: Plenum Press, pp. 113-181.
Fuhrman, J.A., and Suttle, C.A. (1993) Viruses in marine planktonic systems. Oceanography 6: 50-62.
Gasol, J.M., and del Giorgio, P.A. (2000) Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Sci Mar 64: 197-224.
Hahn, M.W., and Hofle, M.G. (2001) Grazing of protozoa and its effect on populations of aquatic bacteria. FEMS Microbiol Ecol 35: 113-121.
Harder, T., Lau, C.K.S., Dobretsov, S., Fang, T.K., and Qian, P.-Y. (2003) A distinctive epibiotic bacterial community on the soft coral Dendronephthya sp. and antibacterial activity of coral tissue extracts suggest a chemical mechanism against bacterial epibiosis. FEMS Microbiol Ecol 43: 337-347.
Hewson, I., Vargo, G.A., and Fuhrman, J.A. (2003) Bacterial diversity in shallow oligotrophic marine benthos and overlying waters: effects of virus infection, containment, and nutrient enrichment. Microb Ecol 46: 322-336.
Horner-Devine, M.C., Carney, K.M., and Bohannan, B.J.M. (2004) An ecological perspective on bacterial biodiversity. Proc R Soc Lond B Biol Sci 271: 113-122.
Jurgens, K., and Gude, H. (1994) The potential importance of grazing-resistant bacteria in planktonic systems. Mar Ecol Prog Ser 112: 169-188.
Jurgens, K., Pernthaler, J., Schalla, S., and Amann, R. (2002) Morphological and compositional changes in a planktonic bacterial community in response to enhanced protozoan grazing. Appl Environ Microbiol 65: 1241-1250.
Kirchman, D., K'nees, E., and Hodson, R. (1985) Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Appl Environ Microbiol 49: 599-607.
Knap, A., Michaels, A., Close, A., Ducklow, H., and Dickson, A. (1996) Protocols for the Joint Global Ocean Flux Study (JGOFS) core measurements. JGOFS report No. 19.
Lebaron, P., Servais, P., Troussellier, M., Courties, C., Vives-Rego, J., Muyzer, G., et al. (1999) Changes in bacterial community structure in seawater mesocosms differing in their nutrient status. Aquat Microb Ecol 19: 255-267.
Middelboe, M., and Lyck, P. (2002) Regeneration of dissolved organic matter by viral lysis in marine microbial communities. Aquat Microb Ecol 27: 187-194.
Middelboe, M., Jorgensen, N.O.G., and Kroer, N. (1996) Effects of viruses on nutrient turnover and growth efficiency of noninfected marine bacterioplankton. Appl Environ Microbiol 62: 1991-1997.
Muyzer, G., de Waal, E.C., and Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59: 695-700.
Noble, R.T., and Fuhrman, J.A. (1999) Breakdown and microbial uptake of marine viruses and other lysis products. Aquat Microb Ecol 20: 1-11.
Pernthaler, J. (2005) Predation on prokaryotes in the water column and its ecological implications. Nat Rev Microbiol 3: 537-546.
Pernthaler, J., and Amann, R. (2005) Fate of heterotrophic microbes in pelagic habitats: focus on populations. Microbiol Mol Biol Rev 69: 440-461.
Piccini, C., Conde, D., Alonso, C., Sommaruga, R., and Pernthaler, J. (2006) Blooms of single bacterial species in a coastal lagoon of the southwestern Atlantic Ocean. Appl Environ Microbiol 72: 6560-6568.
Schwalbach, M.S., Hewson, I., and Fuhrman, J.A. (2004) Viral effects on bacterial community composition in marine plankton mesocosms. Aquat Microb Ecol 34: 117-127.
Simek, K., Pernthaler, J., Weinbauer, M.G., Hornak, K., Dolan, J., Nedoma, J., et al. (2001) Changes in bacterial community composition, dynamics and viral mortality rates associated with enhanced flagellate grazing in a meso-eutrophic reservoir. Appl Environ Microbiol 67: 1723-1733.
Simek, K., Nedoma, J., Penthaler, J., Posch, T., and Dolan, J.R. (2002) Altering the balance between bacterial production and protistan bacterivory triggers shifts in freshwater bacterial community composition. Antonie Van Leeuwenhoek 81: 453-463.
Simek, K., Weinbauer, M.G., Hornak, K., Jezbera, J., Nedoma, J., and Dolan, J.R. (2007) Grazer and virus-induced mortality of bacterioplankton accelerates development of Flectobacillus populations in a freshwater community. Environ Microbiol 9: 789-800.
Sogin, M.L., Morrison, H.G., Huber, J.A., Welch, D.M., Huse, S.M., Neal, P.R., et al. (2006) Microbial diversity in the deep sea and the underexplored 'rare biosphere'. Proc Natl Acad Sci USA 103: 12115-12120.
Suttle, C.A. (2005) Viruses in the sea. Nature 437: 356-361.
Suzuki, M.T. (1999) Effect of protistan bacterivory on coastal bacterioplankton diversity. Aquat Microb Ecol 20: 261-272.
Thingstad, T.F. (2000) Elements of a theory for the mechanisms controlling abundance, diversity, and biogeochemical role of lytic bacterial viruses in aquatic systems. Limnol Oceanogr 45: 1320-1328.
Thingstad, T.F., and Lignell, R. (1997) Theoretical models for the control of bacterial growth rate, abundance, diversity and carbon demand. Aquat Microb Ecol 13: 19-27.
Venter, J.C., Remington, K., Heidelberg, J.F., Halpern, A.L., Rusch, D., Eisen, J.A., et al. (2004) Environmental genome shotgun sequencing of the Sargasso Sea. Science 304: 66-74.
Weinbauer, M.G. (2004) Ecology of prokaryotic viruses. FEMS Microbiol Rev 28: 127-181.
Weinbauer, M., Nedoma, J., Christaki, U., and Simek, K. (2003) Comparing the effects of resource enrichment and grazing on viral production in a meso-eutrophic reservoir. Aquat Microb Ecol 31: 137-144.
Weinbauer, M.G., Hornak, K., Jezbera, J., Nedoma, J., Dolan, J.R., and Simek, K. (2007) Synergistic and antagonistic effects of viral lysis and protistan grazing on bacterial biomass, production and diversity. Environ Microbiol 9: 777-788.
Whitman, W.B., Coleman, D.C., and Wiebe, W.J. (1998) Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 95: 6578-6583.
Wilcox, R.M., and Fuhrman, J.A. (1994) Bacterial viruses in coastal seawater: lytic rather than lysogenic production. Mar Ecol Prog Ser 114: 35-45.
Winter, C., Smit, A., Herndl, G.J., and Weinbauer, M.G. (2004) Impact of virioplankton on archaeal and bacterial community richness as assessed in seawater batch cultures. Appl Environ Microbiol 70: 804-813.
Wommack, K.E., and Colwell, R.R. (2000) Virioplankton: viruses in aquatic ecosystem. Microbiol Mol Biol Rev 70: 69-114.
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Language: | English.
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Document Type: | Research article.
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Journal Subset: | Life Sciences.
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ISSN: | 1462-2912
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NLM Journal Code: | dxc, 100883692
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