BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.
Simao, Felipe A. +; Waterhouse, Robert M. +; Ioannidis, Panagiotis; Kriventseva, Evgenia V.; Zdobnov, Evgeny M. *
[Miscellaneous Article]
Bioinformatics.
31(19):3210-3212, October 01, 2015.
(Format: HTML, PDF)
Motivation: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.
Results: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.
Availability and implementation: Software implemented in Python and datasets available for download from http://busco.ezlab.org.
Contact: [email protected]
Supplementary information: Supplementary data are available at Bioinformatics online.
(C) Copyright Oxford University Press 2015.