Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol.
Han, Dong-Wook 1; Kim, Hak Hee 1; Lee, Mi Hee 2; Baek, Hyun Sook 1; Lee, Kwon-Yong 3; Hyon, Suong-Hyu 4; Park, Jong-Chul 1,2,*
[Article]
Biotechnology Letters.
27(9):655-660, May 2005.
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Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 [mu]g GTP ml-1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.
(C)2005 Kluwer Academic Publishers