Plasma Exosome MicroRNA Profiling Unravels a New Potential Modulator of Adiponectin Pathway in Diabetes: Effect of Glycemic Control.
Santovito, Donato *; De Nardis, Velia *; Marcantonio, Pamela; Mandolini, Claudia; Paganelli, Camilla; Vitale, Elita; Buttitta, Fiamma; Bucci, Marco; Mezzetti, Andrea; Consoli, Agostino; Cipollone, Francesco
[Report]
Journal of Clinical Endocrinology & Metabolism.
99(9):E1681-E1685, September 2014.
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Context: Type 2 diabetes is a chronic disease characterized by inadequate [beta]-cell response to the progressive insulin resistance. MicroRNAs (miRNAs) are short, endogenous, noncoding RNAs representing a class of powerful gene expression modulators. Previous population studies observed a modulation of circulating miRNAs in diabetic patients; however, few data are presently available on miRNA modulation in diabetic patients naive to pharmacological treatment as well as the effect of glycemic control on this.
Objective: We aimed at studying circulating miRNA expression in diabetic patients naive to treatment and at investigating the influence on this of glycemic control.
Design: This was a case-control study.
Participants: Eighteen treatment-naive diabetic patients with poor metabolic control and 12 control patients participated in the study.
Main Outcome Measures: Wide miRNA expression profiling was performed, and the expression of miRNAs found to be dysregulated was then validated by quantitative RT-PCR. Finally, algorithm-identified putative miRNA targets were evaluated by quantitative RT-PCR and ELISA.
Results: In diabetic patients, microarray analysis showed that four miRNAs are increased, whereas 21 miRNAs are decreased. Quantitative RT-PCR validation confirmed the significant up-regulation of miR-326 (P = .004) and down-regulation of let-7a (P < .001) and let-7f (P = .003). Notably, an inverse negative correlation was found between circulating miR-326 and its putative target adiponectin (p = -0.479, P = .009). After 12 months of antidiabetic treatment, quantitative RT-PCR data analysis showed that miR-326 levels were unaffected, whereas the levels of let-7a and let-7f were significantly increased.
Conclusions: Treatment-naive, poorly controlled diabetic patients show a significant dysregulation of miRNAs involved in the regulation of the adiponectin pathway, a phenomenon that may be reversed, at least in part, by improved glycemic control.
Copyright (C) 2014 by The Endocrine Society