Information de reference pour ce titreAccession Number: | 00001833-200421060-00003.
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Author: | Gardella, Carolyn M.D., M.P.H. 1; Riley, Donald E. Ph.D. 2; Hitti, Jane M.D., M.P.H. 1; Agnew, Kathy B.S. 1; Krieger, John N. M.D. 1; Eschenbach, David M.D. 1
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Institution: | (1)Department of Obstetrics and Gynecology, University of Washington, and VA Medical Center, Seattle, Washington (2)Department of Urology, University of Washington, and VA Medical Center, Seattle, Washington.
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Title: | |
Source: | American Journal of Perinatology. 21(6):319-323, 2004.
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Abstract: | Our objective was to identify bacterial species present in culture-negative but 16S rDNA-positive amniotic fluid samples from women in preterm labor. Amniotic fluid from 69 women in preterm labor was cultured and examined for the proinflammatory cytokine interleukin-6 (IL-6). Polymerase chain reaction technology was used to detect highly conserved bacterial ribosomal DNA sequences (16S rDNAs). As previously reported, 16S rDNAs were identified in 15 (94%) of 16 culture-positive amniotic fluid samples, in 5 (36%) of 14 culture-negative samples with elevated IL-6, and in 1 (3%) of 39 culture-negative samples with low IL-6 levels. Direct sequencing was performed of 16S rDNAs from the 5 culture-negative amniotic fluid specimens with elevated IL-6, followed by database searches and phylogenetic analyses. The bacterial sequences identified included: two Leptotrichia sanguinegens, one human oral bacterium A33, one Fusobacterium nucleatum, and one Ureaplasma urealyticum. Identification and sequencing of 16S rDNAs in amniotic fluid is a promising technique to identify bacterial species associated with elevated IL-6 levels in culture-negative amniotic fluid that may contribute to the etiology of premature labor.
Copyright (C) 2004 by Thieme
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Author Keywords: | 16S rDNA; PCR; preterm labor; amniotic fluid; intra-amniotic infection.
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Language: | English.
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Document Type: | Original Article.
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Journal Subset: | Clinical Medicine.
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ISSN: | 0735-1631
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NLM Journal Code: | aa3, 8405212
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