Genome-wide identification of NBS resistance genes in Populus trichocarpa.
Kohler, Annegret 1; Rinaldi, Cecile 1; Duplessis, Sebastien 1; Baucher, Marie 2; Geelen, Danny 3; Duchaussoy, Frederic 1; Meyers, Blake C. 4; Boerjan, Wout 5,6; Martin, Francis 1
[Article]
Plant Molecular Biology.
66(6):619-636, April 2008.
(Format: HTML, PDF)
As the largest class of disease resistance R genes, the genes encoding nucleotide binding site and leucine-rich repeat proteins (ldquo;NBS-LRRgenes") play a critical role in defending plants from a multitude of pathogens and pests. The diversity of NBS-LRR genes was examined in the Populus trichocarpa draft genome sequence. The NBS class of genes in this perennial tree is large and diverse, comprised of ~400 genes, at least twice the complement of Arabidopsis. The NBS family can be divided into multiple subfamilies with distinct domain organizations. It includes 119 Coiled-Coil-NBS-LRR genes, 64TIR-NBS-LRR genes, 34 BED-finger-NBS-LRR, and both truncated and unusual NBS- andNBS-LRR-containing genes. The transcripts of only 34 NBS-LRR genes were detected in rust-infected and non-infected leaves using a whole-genome oligoarray. None showed an altered expression two days post inoculation.
(C)2008 Kluwer Academic Publishers