Information de reference pour ce titreAccession Number: | 00000422-200407000-00003.
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Author: | Barletta, Janet M. PhD; Edelman, Daniel C. MS; Constantine, Niel T. PhD
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Institution: | Pathology Department, University of Maryland Baltimore
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Title: | |
Source: | American Journal of Clinical Pathology. 122(1):20-27, July 01, 2004.
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Abstract: | Presently, the assay that attains maximal sensitivity and dynamic range of HIV-1 viral copy number (50 copies per milliliter) is nucleic acid amplification of HIV RNA in plasma. Enzyme-linked immunosorbent assay (ELISA) methods for quantification of HIV-1 p24 antigen have been relatively insensitive. In this report, we show data that indicate real-time immuno-polymerase chain reaction (IPCR), a combination of the ELISA and PCR techniques, is more sensitive for HIV-1 p24 antigen detection than other currently reported methods. When derived from an IPCR standard curve, a dose response was observed from patient samples with known viral loads diluted within a 3-log range (1.68-6,514 viral RNA copies per milliliter). IPCR detected 42% (22/52) of patient samples that had fewer than 50 viral RNA copies per milliliter by reverse transcriptase-PCR. IPCR shows the potential to become the most analytically sensitive test available for determination of HIV-1 viral load by the detection of HIV-1 p24 antigen.
Copyright (C) Oxford University Press 2015
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Author Keywords: | Immuno-PCR; IPCR; Polymerase chain reaction; p24 antigen; HIV-1; Viral load; ELISA; Enzyme-linked immunosorbent assay; Real-time PCR.
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Language: | English.
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Document Type: | Articles.
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ISSN: | 0002-9173
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NLM Journal Code: | 3fk, 0370470
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DOI Number: | https://dx.doi.org/10.1309/529T2...- ouverture dans une nouvelle fenêtre
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