Information de reference pour ce titreAccession Number: | 00008572-200707020-00013.
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Author: | von Dahl, Caroline C. 1; Winz, Robert A. 1,+; Halitschke, Rayko 1,++; Kuhnemann, Frank 2,[S]; Gase, Klaus 1; Baldwin, Ian T. 1,*
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Institution: | (1)Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, Hans-Knoll-Strasse 8, 07745 Jena, Germany (2)Institut fur Angewandte Physik, Universitat Bonn, Wegelerstrasse 8, 53115 Bonn, Germany
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Title: | |
Source: | Plant Journal. 51(2):293-307, July 2007.
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Abstract: | Summary: Caterpillar-induced ethylene emissions play an important role in plant-herbivore interactions. The ethylene burst that ensues after attack exceeds wound-induced ethylene emissions, but the mechanisms responsible remain unknown. Adding larval oral secretions (OS) to wounds mimics this ethylene burst. We demonstrate that fatty acid-amino acid conjugates are the responsible elicitors in Manduca sexta OS, and identify genes that are important in OS-elicited ethylene biosynthesis and perception in the larvae's host, Nicotiana attenuata, by examining the consequences of gene silencing on OS-elicited ethylene emissions, as quantified by photo-acoustic spectroscopy. OS elicitation increased transcript accumulation of ACC synthase (ACS), virus-induced gene silencing of ACS halved the OS-elicited ethylene release, and ACC supplementation to ACS-silenced plants restored ethylene emissions, demonstrating that ACS activity limits the rate of release. Silencing three wound- or OS-elicited ACC oxidase (ACO) genes with an ACO consensus fragment abolished the OS-elicited ethylene release. Virus-induced gene silencing of each ACO individually revealed that only NaACO2a and NaACO3 regulate the OS-elicited ethylene release. Transforming plants with various etr1-1 constructs rendered them differentially 'deaf' to ethylene, and dramatically increased the OS-elicited ethylene burst, largely without regulating the transcripts of biosynthetic genes. The volume of the OS-elicited ethylene 'scream' was proportional to the plant's deafness, as determined by 1-MCP treatments. We conclude that the OS-elicited ethylene burst is tuned by a tag-team of transcriptional responses and ethylene perception. Ethylene signaling is shown to be essential in regulating two traits that are important in the N. attenuata-M. sexta interaction: OS-induced nicotine levels and floral longevity.
Copyright (C) 2007 Blackwell Publishing Ltd.
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Author Keywords: | ethylene biosynthesis; ACO transcript accumulation; elicitor; ethylene perception; feedback regulation; induced defense.
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Language: | English.
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Document Type: | ORIGINAL ARTICLE.
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Journal Subset: | Life Sciences.
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ISSN: | 0960-7412
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NLM Journal Code: | bru, 9207397
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