Information de reference pour ce titreAccession Number: | 00006056-199603070-00028.
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Author: | Klingelhutz, Aloysius J.; Foster, Scott A.; McDougall, James K.
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Institution: | (Klingelhutz, Foster, McDougall) Program in Cancer Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA. (McDougall) Department of Pathology, University of Washington, Seattle, Washington 98195, USA.
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Title: | Telomerase activation by the E6 gene product of human papillomavirus type 16.[Letter]
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Source: | Nature. 380(6569):79-82, March 7, 1996.
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Abstract: | ACTIVATION of telomerase, a ribonucleoprotein complex that synthesizes telomere repeat sequences *RF 1-4*, is linked to cell immortalization and is characteristic of most cell lines and tumours [4,5-8]. Here we show that expression of the human papillomavirus type 16 (HPV-16) E6 protein activates telomerase in early-passage human keratinocytes and mammary epithelial cells. This activation was observed in cells pre-crisis, that is, before they became immortal, and occurred within one passage of retroviral infection with vectors expressing HPV-16 E6. Studies using HPV-16 E6 mutants showed that there was no correlation between the ability of the mutants to activate telomerase and their ability to target p53 for degradation, suggesting that telomerase activation by HPV-16 E6 is p53 independent. Keratinocytes expressing wild-type HPV-16 E6 have an extended lifespan, but do not become immortal [9], indicating that telomerase activation and E6-mediated degradation of p53 are insufficient for their immortalization. These results show that telomerase activation is an intrinsic, but insufficient, component of transformation by HPV.
(C) 1996 Macmillan Magazines Ltd.
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Language: | English.
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Document Type: | LETTER TO NATURE.
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Journal Subset: | Life Sciences. Physical Science & Engineering.
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ISSN: | 0028-0836
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NLM Journal Code: | 0410462, nsc
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