Ceftazidime-hydrolysing CTX-M-15 extended-spectrum [beta]-lactamase (ESBL) in Poland.
Baraniak, Anna 1; Fiett, Janusz 1; Hryniewicz, Waleria 1; Nordmann, Patrice 2; Gniadkowski, Marek 1,*
[Report] Journal of Antimicrobial Chemotherapy. 50(3):393-396, September 2002.

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The CTX-M-15 extended-spectrum [beta]-lactamase (ESBL) was recently identified in Enterobacteriaceae isolates in India, and demonstrated significant hydrolytic activity against ceftazidime, in contrast to the majority of CTX-M enzymes. CTX-M-15 differs from CTX-M-3, which is one of the most prevalent ESBLs in Poland, by only a single amino acid change (Asp-240->Gly). Three cefotaxime- and ceftazidime-resistant Enterobacteriaceae isolates, recovered during 1998-2000 in two Polish hospitals, were found to produce CTX-M-15. Similar to those from India, the isolates contained the ISEcp1 insertion sequence located upstream of the blaCTX-M-15 gene, which has been recently demonstrated to mobilize 3'-adjacent genes to transfer between DNA replicons. However, its different position with respect to the [beta]-lactamase gene indicated the independent selection of the ESBL gene in the two countries.

(C) Copyright Oxford University Press 2002.