HDAC2 Suppresses IL17A-Mediated Airway Remodeling in Human and Experimental Modeling of COPD.
Lai, Tianwen MD, PhD a,b; Tian, Baoping MD, PhD a; Cao, Chao MD, PhD a; Hu, Yue MD, PhD a; Zhou, Jiesen MD, PhD a; Wang, Yong MD, PhD a; Wu, Yanping MD, PhD a; Li, Zhouyang MD, PhD a; Xu, Xuchen MD, PhD a; Zhang, Min BA a; Xu, Feng MD, PhD a; Cao, Yuan MD a; Chen, Min MD a,b; Wu, Dong MD b; Wu, Bin MD b; Dong, Chen PhD c; Li, Wen MD, PhD a; Ying, Songmin PhD a; Chen, Zhihua PhD a; Shen, Huahao MD, PhD, FCCP a,d,*
[Article]
Chest.
153(4):863-875, April 2018.
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BACKGROUND: Although airway remodeling is a central feature of COPD, the mechanisms underlying its development have not been fully elucidated. The goal of this study was to determine whether histone deacetylase (HDAC) 2 protects against cigarette smoke (CS)-induced airway remodeling through IL-17A-dependent mechanisms.
METHODS: Sputum samples and lung tissue specimens were obtained from control subjects and patients with COPD. The relationships between HDAC2, IL-17A, and airway remodeling were investigated. The effect of HDAC2 on IL-17A-mediated airway remodeling was assessed by using in vivo models of COPD induced by CS and in vitro culture of human bronchial epithelial cells and primary human fibroblasts exposed to CS extract, IL-17A, or both.
RESULTS: HDAC2 and IL-17A expression in the sputum cells and lung tissue samples of patients with COPD were associated with bronchial wall thickening and collagen deposition. Il-17a deficiency (Il-17a-/-) resulted in attenuation of, whereas Hdac2 deficiency (Hdac2 /-) exacerbated, CS-induced airway remodeling in mice. IL-17A deletion also attenuated airway remodeling in CS-exposed Hdac2 /- mice. HDAC2 regulated IL-17A production partially through modulation of CD4 T cells during T helper 17 cell differentiation and retinoid-related orphan nuclear receptor [gamma]t in airway epithelial cells. In vitro, IL-17A deficiency attenuated CS-induced mouse fibroblast activation from Hdac2 /- mice. IL-17A-induced primary human fibroblast activation was at least partially mediated by autocrine production of transforming growth factor beta 1.
CONCLUSIONS: These findings suggest that activation of HDAC2 and/or inhibition of IL-17A production could prevent the development of airway remodeling by suppressing airway inflammation and modulating fibroblast activation in COPD.
(C) 2018Elsevier, Inc.
