ROLLED LEAF 9, encoding a GARP protein, regulates the leaf abaxial cell fate in rice.
Yan, Song 1; Yan, Chang-Jie 1; Zeng, Xiu-Hong 1; Yang, Ya-Chun 1; Fang, Yu-Wei 1; Tian, Chun-Yan 1; Sun, Ya-Wei 1; Cheng, Zhu-Kuan 2; Gu, Ming-Hong 1
[Article] Plant Molecular Biology. 68(3):239-250, October 2008.

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Leaves, the collective organ produced by the shoot apical meristem (SAM), are polarized along their adaxial-abaxial axis. In this study, we characterized two rice (Oryza sativa) allelic rolled-leaf mutants, rolled leaf 9-1(rl9-1) and rl9-2, which display very similar phenotypes with completely adaxialized leaves and malformed spikelets. We cloned the RL9 gene by way of a map-based cloning strategy. Molecular studies have revealed that RL9 encodes a GARP protein, an orthologue of Arabidopsis KANADIs. RL9 is mainly expressed in roots, leaves, and flowers. The transient expression of a RL9-GFP (green fluorescent protein) fusion protein has indicated that RL9 protein is localized in the nucleus, suggesting that RL9 acts as a putative transcription factor.

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