Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients.
Davidsson, Pia CA; Westman-Brinkmalm, Ann; Nilsson, Carol L. 1; Lindbjer, Maria; Paulson, Linda; Andreasen, Niels 2; Sjogren, Magnus; Blennow, Kaj
13(5):611-615, April 16, 2002.
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: By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, [beta]-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.
(C) 2002 Wolters Kluwer Health | Lippincott Williams & Wilkins