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: Vaccination strategies based on repeated injections of NY-ESO-1 protein formulated in ISCOMATRIX particles (NY-ESO-1 ISCOMATRIX) have shown to elicit combined NY-ESO-1 specific antibody and T cell responses. However, it remains unclear whether heterologous prime-boost strategies based on the combination with NY-ESO-1 ISCOMATRIX with different NY-ESO-1 boosting reagents could be used to increase NY-ESO-1 CD8 or CD4 T cell responses. To address this question, we carried out a randomized clinical trial in 39 high-risk, resected melanoma patients vaccinated with NY-ESO-1 ISCOMATRIX, and then boosted with repeated injections of either recombinant fowlpox virus encoding full length NY-ESO-1 (rF-NY-ESO-1) (Arm A) or NY-ESO-1 ISCOMATRIX alone (Arm B). We have comprehensively analyzed NY-ESO-1 specific T cells and B cells response in all patients before and after vaccination for a total of seven time points per patient. NY-ESO-1 ISCOMATRIX alone elicited a strong NY-ESO-1 specific CD4 T cell and antibody response, which was maintained by both regiments at similar levels. However, CD8 T cell responses were significantly boosted in 3 out of 18 patients in Arm A after the first rF-NY-ESO-1 injection and such responses were maintained until the end of the trial, while no patients in Arm B showed similar CD8 T cell responses. In addition, our results clearly identified immunodominant regions in the NY-ESO-1 protein: NY-ESO-179-102 and NY-ESO-1115-138 for CD4 T cells and NY-ESO-185-108 for CD8 T cells in a large proportion of vaccinated patients. These regions of NY-ESO-1 protein should be considered in future clinical trials as immunodominant epitopes.

What's New?: Cancer vaccines using genetically-engineered viruses have not been as successful as originally hoped. In this study, the authors asked whether first priming the immune system with antigen alone might improve the success of a viral vector. Melanoma patients were given priming injections of an antigen called NY-ESO-1 plus ISCOMATRIX adjuvant. Several patients who then received injections of a fowlpox virus encoding NY-ESO-1 had improved CD8 T-cell responses, compared to no improvement in patients who simply received more of the isolated antigen.

(C) 2015 John Wiley & Sons, Ltd