Cell Surface Recycling of Internalized Antigen Permits Dendritic Cell Priming of B Cells.
Bergtold, Amy 1; Desai, Dharmesh D. 2; Gavhane, Anamika 2; Clynes, Raphael 2,*
[Article]
Immunity.
23(5):503-514, November 2005.
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Summary: Dendritic cells process internalized antigens to present degradative products on MHC for TCR recognition. Because antigen-exposed DCs also induce humoral immunity, DCs must also retain antigen in its native state for the engagement of BCR on B cells. Here, we demonstrate that antigen endocytosed by the inhibitory Fc receptor, Fc[gamma]RIIB, accesses a nondegradative intracellular vesicular compartment that recycles to the cell surface, enabling interaction of native antigen with BCR on B cells. Immunization with IgG-opsonized, T independent antigens leads to enhanced humoral responses in a Fc[gamma]RIIB and complement dependent manner. IC-loaded DCs trafficking to the splenic marginal zone can prime a T independent response in an Fc[gamma]RIIB-dependent manner. Thus dendritic cells are equipped with both nondegradative and degradative antigen uptake pathways to facilitate antigen presentation to both B and T cells.
(C) 2005Elsevier, Inc.