Gene Transfer of CD40-Ligand Induces Autologous Immune Recognition of Chronic Lymphocytic Leukemia B Cells.
Kato, Kazunori; Cantwell, Mark J.; Sharma, Sanjai; Kipps, Thomas J.
[Miscellaneous Article] Journal of Clinical Investigation. 101(5):1133-1141, March 1, 1998.

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CD40-CD40-ligand (CD154) interactions play a critical role in immune activation. Using replication defective adenovirus encoding mouse CD154 (Ad-CD154), we modified human chronic lymphocytic leukemia B cells to express a functional ligand for CD40. This not only induces expression of immune accessory molecules on the infected cell, but also allows it to trans-activate noninfected bystander leukemia B cells. Also, factors that impair the antigen-presenting capacity of leukemia B cells are downmodulated. Ad-CD154-infected leukemia cells are highly effective stimulators in mixed lymphocyte reactions and can induce generation of cytotoxic T lymphocytes specific for autologous nonmodified leukemia cells. As such, Ad-CD154 can induce a host antileukemia response that may have therapeutic potential. (J. Clin. Invest. 1998. 101:1133-1141.)

Copyright (C) 1998 The American Society for Clinical Investigation, Inc.