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A combination of intracellular recording and fluoromet-ric measurements of cytosolic calcium ([Ca2 ]i) was used to locate changes in [Ca2 ], induced by the specific meta-botropic glutamate receptor (mGluR) agonist trans-D,L-l-amino-1,3-cyclopentanedicarboxylic acid (t-ACPD), in Purkinje cells of rat cerebellar slices. Under voltage-clamp conditions, application of t-ACPD (100 [mu]M) induced an inward current accompanied by a large increase in [Ca2 ] located primarily in the soma but also, to a lesser degree, in restricted parts of the dendrites. In contrast, elevations of [Ca2 ]; associated with calcium spikes were confined to the dendrites and inward currents of a similar amplitude induced by (RS)-[alpha]-amino-3-hydroxy-5-me-thyl-4-isoxazolepropionic acid (AMPA), an agonist of ionotropic glutamate receptors, did not raise [Ca2 ]i.

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